Strain Data Sheet

RBRC09499

Strain Information

Image
BRC No.RBRC09499
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129R1-Eif3b<Gt(GT529)Skmz>
Former Common namep116, B6.129R1-Tg(p116)
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Embryonic lethality of mutant mice deficient in the p116 gene.
Koyanagi-Katsuta R, Akimitsu N, Hamamoto H, Arimitsu N, Hatano T, Sekimizu K
J. Biochem., 131, 833-837 (2002). 12038979

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Eif3b
MGI:106478		eukaryotic translation initiation factor 3, subunit B5Eif3b<Gt(GT529)Skmz>
MGI:5636799		eukaryotic translation initiation factor 3, subunit B; gene trap mutation GT529, Kazuhisa Sekimizu
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)5En2
Ires internal ribosomal entry site (EMCV)5
lacZ beta-galactosidase (E. coli)5
neo neomycin resistance gene (E. coli)5
SV40 polyA signal5

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryonic lethality before implantation, complete penetrance(MP:0011094)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse En2 splice acceptor, E. coli lacZ, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli neo, SV40 polyadenylation site
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biochem., 131, 833-837 (2002).
    DepositorKazuhisa Sekimizu (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data