Strain Data Sheet

RBRC09500

Strain Information

Image
BRC No.RBRC09500
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2-Wbp2nl<tm1b(EUCOMM)Osb>
Former Common namePAWP KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation.
Satouh Y, Nozawa K, Ikawa M
Biol. Reprod., 93(4):94 (2015). 26377222

Health Report

Examination Date / Room / Rack2024/11/18Room:4-ARack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)15En2
Frt yeast FRT (flippase recombination target) site15Frt
Ires internal ribosomal entry site (EMCV)15
Wbp2nl
MGI:1921966		WBP2 N-terminal like15Wbp2nl<tm1b(EUCOMM)Osb> targeted mutation 1b, Research Institute for Microbial Diseases, Osaka University
lacZ beta-galactosidase (E. coli)15
loxP phage P1 loxP15loxP
SV40 polyA15

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site, En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyA, phage P1 loxP site, mouse Wbp2nl genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 93(4):94 (2015). Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. This strain was established by the DEPOSITOR by using knockout ES cells of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the ES cells of the strain.The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data