Strain Data Sheet

RBRC09525

Strain Information

Image
BRC No.RBRC09525
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ins1<em1(cre)Utr>
Former Common nameI2AC mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice.
Yoshikazu Hasegawa, Yoshikazu Hoshino, Abdelaziz E Ibrahim, Kanako Kato, Yoko Daitoku, Yoko Tanimoto, Yoshihisa Ikeda, Hisashi Oishi, Satoru Takahashi, Atsushi Yoshiki, Ken-Ichi Yagami, Hiroyoshi Iseki, Seiya Mizuno, Fumihiro Sugiyama
Exp. Anim., 65(3):319-27 (2016). 27053096

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:CSentinel mouse program
2024/04/08Room:3-ARack:DSentinel mouse program
2024/02/19Room:4-ARack:CSentinel mouse program
2024/01/09Room:3-ARack:ASentinel mouse program
2023/11/20Room:4-ARack:CSentinel mouse program
2023/11/13Room:4-ARack:CAdditional tests
2023/10/10Room:3-ARack:ASentinel mouse program
2023/08/21Room:4-ARack:CSentinel mouse program
2023/08/07Room:4-ARack:CAdditional tests
2023/07/10Room:3-ARack:ASentinel mouse program
2023/05/22Room:4-ARack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin polyA19HBB2
Ins1
MGI:96572		insulin I19Ins1<em1(cre)Utr> endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center
  • diabetes mellitus, permanent neonatal 4(MedGEN)

  • hyperproinsulinemia(MedGEN)

  • maturity-onset diabetes of the young type 10(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young(DisGeNET)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 1 diabetes mellitus 2(MedGEN)
    • P2A porcine teschovirus-1 2A peptide19P2A
    cre Phage P1 Cre recombinase19cre
    nls SV40 large T antigen nuclear localization signal (NLS)19nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), Simian virus 40 nuclear localization signal (NLS), bacteriophage P1 Cre recombinase, rabbit beta globin polyA
    Research applicationCre/loxP system
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.)
    DepositorFumihiro Sugiyama (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2018

    BRC mice in Publications

    Lai S, Higuchi T, Tsuda M, Sugiyama Y, Morisawa K, Taniguchi T, Sakamoto S.
    NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.
    Sci Rep 12(1) 8837(2022) 35614067
    Mostafa D, Yanagiya A, Georgiadou E, Wu Y, Stylianides T, Rutter GA, Suzuki T, Yamamoto T.
    Loss of β-cell identity and diabetic phenotype in mice caused by disruption of CNOT3-dependent mRNA deadenylation.
    Commun Biol 3(1) 476(2020) 32859966