Strain Information | |
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Image | |
BRC No. | RBRC09532 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129-Hprt<tm1(CAG-eGFP-NLS)Koba> |
Former Common name | Hprt-eGFP, Momiji mouse |
H-2 Haplotype | |
ES Cell line | BPES11[carries Hprt<b-m3>] |
Background strain | |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References | Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naive from primed pluripotent stem cells. Kobayashi S, Hosoi Y, Shiura H, Yamagata K, Takahashi S, Fujihara Y, Kohda T, Okabe M, Ishino F Development, 143(16):2958-64 (2016). 27471261Live imaging of X chromosome inactivation and reactivation dynamics. Kobayashi S Dev. Growth Differ. (2017). 28635043Live Imaging of X-Chromosome Inactivation and Reactivation Kinetics. Kobayashi S Methods Mol Biol., 1861:73-89 (2018). 30218361Visualization of X chromosome reactivation in mouse primordial germ cells in vivo. Haramoto Y, Sakata M, Kobayashi S Biol Open (2021) 10 (4): bio058602. 33913476 |
Health Report | |
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Examination Date / Room / Rack | 2024/06/10Room:3-3Rack:DSentinel mouse program 2024/03/11Room:3-3Rack:DSentinel mouse program 2023/12/11Room:3-3Rack:DSentinel mouse program 2023/09/11Room:3-3Rack:DSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | X | EGFP | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
Frt | yeast FRT (flippase recombination target) site | X | Frt | ||||
Hprt MGI:96217 | hypoxanthine guanine phosphoribosyl transferase | X | Hprt<tm1(CAG-eGFP-NLS)Koba> | targeted mutation 1, Shin Kobayashi | |||
loxP | phage P1 loxP | X | loxP | ||||
nls | SV40 large T antigen nuclear localization signal (NLS) | X | nls |
Phenotype | |
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Phenotype annotation from literatures by Mammalian phenotype ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP cDNA, NLS derived from the human MBD1 cDNA, mouse Hprt genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 143(16):2958-64 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shin Kobayashi (National Institute of Advanced Industrial Science and Technology) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Guo Q, Kouyama-Suzuki E, Shirai Y, Tabuchi K. Jun N-Terminal Kinase Inhibitor Suppresses CASK Deficiency-Induced Cerebellar Granular Cell Death in MICPCH Syndrome Model Mice. Cells 14(10) (2025) 40422253 |
Mori T, Zhou M, Kunugitani K, Akatsuka T, Yoshida Y, Kouyama-Suzuki E, Kobayashi S, Shirai Y, Tabuchi K. The Competitive Loss of Cerebellar Granule and Purkinje Cells Driven by X-Linked Mosaicism in a Female Mouse Model of CASK-Related Disorders. Cells 14(10) (2025) 40422238 |