Strain Data Sheet

RBRC09534

Strain Information

Image
BRC No.RBRC09534
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pgk1<tm1(CAG-eGFP-NLS,PGK-Neo)Koba>
Former Common namePgk1-eGFP (Neo+) mouse, Momiji mouse
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naive from primed pluripotent stem cells.
Kobayashi S, Hosoi Y, Shiura H, Yamagata K, Takahashi S, Fujihara Y, Kohda T, Okabe M, Ishino F
Development, 143(16):2958-64 (2016). 27471261

Live imaging of X chromosome inactivation and reactivation dynamics.
Kobayashi S
Dev. Growth Differ. (2017). 28635043

Live Imaging of X-Chromosome Inactivation and Reactivation Kinetics.
Kobayashi S
Methods Mol Biol., 1861:73-89 (2018). 30218361

Health Report

Examination Date / Room / Rack2023/10/10Room:3-ARack:HSentinel mouse program
2023/07/10Room:3-ARack:HSentinel mouse program
2023/04/10Room:3-ARack:HSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)XEGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Frt yeast FRT (flippase recombination target) siteXFrt
Frt yeast FRT (flippase recombination target) siteXFrt
MBD1 methyl-CpG binding domain protein 1 (human)XMBD1
Pgk1
MGI:97555		phosphoglycerate kinase 1XPgk1<tm1(CAG-eGFP-NLS,PGK-Neo)Koba> targeted mutation 1, Shin Kobayashi
  • glycogen storage disease due to phosphoglycerate kinase 1 deficiency(MedGEN)
    • loxP phage P1 loxPXloxP
    loxP phage P1 loxPXloxP
    neo neomycin resistance gene (E. coli)X mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP cDNA, human MBD1 cDNA, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), Streptomyces fradiase neomycin phosphotransferase genomic DNA, mouse Pgk1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 143(16):2958-64 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShin Kobayashi (National Institute of Advanced Industrial Science and Technology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data