Strain Data Sheet

RBRC09537

Strain Information

Image
BRC No.RBRC09537
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pgk1<tm1(CAG-mcherry-NLS,PGK-Puro)Koba>
Former Common namePgk1-RED (Puro+) mouse, Momiji mouse
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naive from primed pluripotent stem cells.
Kobayashi S, Hosoi Y, Shiura H, Yamagata K, Takahashi S, Fujihara Y, Kohda T, Okabe M, Ishino F
Development, 143(16):2958-64 (2016). 27471261

Live imaging of X chromosome inactivation and reactivation dynamics.
Kobayashi S
Dev. Growth Differ. (2017). 28635043

Health Report

Examination Date / Room / Rack2023/04/10Room:3-ARack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteXFrt
Frt yeast FRT (flippase recombination target) siteXFrt
MBD1 methyl-CpG binding domain protein 1 (human)XMBD1
Pgk1
MGI:97555		phosphoglycerate kinase 1XPgk1<tm1(CAG-mcherry-NLS,PGK-Puro)Koba> targeted mutation 1, Shin Kobayashi
  • glycogen storage disease due to phosphoglycerate kinase 1 deficiency(MedGEN)
    • loxP phage P1 loxPXloxP
    loxP phage P1 loxPXloxP
    mCherry Red Fluorescent Protein (Discosoma sp.)XmCherry CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
    puro puromycin acyltransferase (Streptomyces alboniger)Xpuro mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. mCherry cDNA, human MBD1 cDNA, Yeast yeast FRT (flippase recombination target) sites, Streptomyces alboniger puromycin-resistance gene, mouse Pgk1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 143(16):2958-64 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShin Kobayashi (National Institute of Advanced Industrial Science and Technology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data