Strain Information | |
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Image | |
BRC No. | RBRC09552 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129-Actb<tm3.1(tetO-GCaMP6)Kftnk> |
Former Common name | tetO-GCaMP6, B6;129-Actb<tm1(tetO-GCaMP6)Kftnk>/Kftnk(tetO-GCaMP6) |
H-2 Haplotype | |
ES Cell line | G0G1 |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References | Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J PLoS One, 7, e51286, (2012). 23240011 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Actb MGI:87904 | actin, beta | 5 | Actb<tm1(tetO-GCaMP6)Kftnk> | targeted mutation 1, Kenji Tanaka | more 1 Diseases | ||
Frt | yeast FRT (flippase recombination target) site | 5 | Frt | ||||
GCaMP6 | RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | 5 | GCaMP6 | E. coli tet operator, cytomegalovirus CMV promoter | |||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
SV40 polyA signal | 5 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Yoshida K, Kato D, Sugio S, Takeda I, Wake H. Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer's disease. Front Cell Neurosci 17 1154196(2023) 38026691 |
Moriya S, Yamashita A, Nishi R, Ikoma Y, Yamanaka A, Kuwaki T. Acute nociceptive stimuli rapidly induce the activity of serotonin and noradrenalin neurons in the brain stem of awake mice. IBRO Rep 7 1-9(2019) 31194165 |