Strain Data Sheet

RBRC09552

Strain Information

Image
BRC No.RBRC09552
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Actb<tm3.1(tetO-GCaMP6)Kftnk>
Former Common nametetO-GCaMP6, B6;129-Actb<tm1(tetO-GCaMP6)Kftnk>/Kftnk(tetO-GCaMP6)
H-2 Haplotype
ES Cell lineG0G1
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals.
Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J
PLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actb
MGI:87904
actin, beta5Actb<tm1(tetO-GCaMP6)Kftnk> targeted mutation 1, Kenji Tanaka
  • autosomal dominant nonsyndromic hearing loss 20(MedGEN)

  • Baraitser-Winter syndrome 1(MedGEN)

  • Baraitser-winter syndrome 2(MedGEN)
  • more 1 Diseases
  • developmental malformations-deafness-dystonia syndrome(MedGEN)
  • Frt yeast FRT (flippase recombination target) site5Frt
    GCaMP6 RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA5GCaMP6 E. coli tet operator, cytomegalovirus CMV promoter
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    SV40 polyA signal5

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Yoshida K, Kato D, Sugio S, Takeda I, Wake H.
    Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer's disease.
    Front Cell Neurosci 17 1154196(2023) 38026691
    Moriya S, Yamashita A, Nishi R, Ikoma Y, Yamanaka A, Kuwaki T.
    Acute nociceptive stimuli rapidly induce the activity of serotonin and noradrenalin neurons in the brain stem of awake mice.
    IBRO Rep 7 1-9(2019) 31194165