Strain Data Sheet
RBRC09592
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC09592 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6-ROSA26Tm(SAEiCE)13Card |
| Former Common name | R26SAEiCE, 13 |
| H-2 Haplotype | |
| ES Cell line | 6NK-7 [C57BL/6N] |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/01/20Room:3-CRack:ESentinel mouse program 2024/10/21Room:3-CRack:ESentinel mouse program 2024/07/22Room:3-CRack:ESentinel mouse program 2024/04/22Room:3-CRack:ESentinel mouse program 2024/01/29Room:3-CRack:EAdditional tests 2024/01/22Room:3-CRack:ESentinel mouse program 2023/10/23Room:3-CRack:ESentinel mouse program 2023/07/24Room:3-CRack:ESentinel mouse program 2023/04/24Room:3-CRack:ESentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| ERT2 | estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A) | 6 | ERT2 | ||||
| En2 | En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence) | 6 | En2 | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Gt(ROSA26)Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA26)Sor | ||||
| iCre | phage P1 codon improved Cre (iCre) | 6 | iCre | ||||
| lox2272 | phage P1 loxP variant | 6 | lox2272 | ||||
| lox71 | mutant lox sequence | 6 | lox71 | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| puro | puromycin acyltransferase (Streptomyces alboniger) | 6 | puro | mouse phosphoglycerate kinase promoter (PGK promoter) | |||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | phage P1 loxP and mutant lox sequence, mouse En2 splice acceptor, human estrogen receptor cDNA(ESR1), phage P1 cre recombinase, mouse PGK promoter, Streptomyces alboniger puromycin resistant gene, mouse PGK polyA signal, yeast FRT (flipase recombination target) sites, E. coli pSP73 vector sequence, mouse ROSA26 genomic sequence |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Masatake Araki (Kumamoto University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |