Strain Data Sheet

RBRC09595

Strain Information

Image
BRC No.RBRC09595
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Keap1<tm2Mym>
Former Common nameKeap1 flox
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Hepatocyte-specific deletion of the keap1 gene activates Nrf2 and confers potent resistance against acute drug toxicity.
Okawa H, Motohashi H, Kobayashi A, Aburatani H, Kensler T W, Yamamoto M
Biochem. Biophys. Res. Commun., 339, 79-88 (2006). 16293230

Genetic analysis of cytoprotective functions supported by graded expression of Keap1.
Taguchi K, Maher J M, Suzuki T, Kawatani Y, Motohashi H, Yamamoto M
Mol Cell Biol., 30, 3016-3026 (2010). 20404090

Health Report

Examination Date / Room / Rack2023/08/28Room:4-BRack:ISentinel mouse program
2023/05/29Room:4-BRack:ISentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)9EGFP
Keap1
MGI:1858732		kelch-like ECH-associated protein 19Keap1<tm2Mym>
MGI:3611748		targeted mutation 2, Masayuki Yamamoto
loxP phage P1 loxP9loxP
loxP phage P1 loxP9loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • decreased susceptibility to xenobiotic induced morbidity/mortality(MP:0009767)

  • lethality at weaning(MP:0008569)

  • postnatal growth retardation(MP:0001732)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 LoxP sites, Splicing accepter, jellyfish EGFP cDNA, mouse Keap1 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Res Commun., 339, 79-88 (2006)., Mol Cell Biol., 30, 3016-3026 (2010).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorMasayuki Yamamoto (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Vallelian F, Buzzi RM, Pfefferlé M, Yalamanoglu A, Dubach IL, Wassmer A, Gentinetta T, Hansen K, Humar R, Schulthess N, Schaer CA, Schaer DJ.
    Heme-stress activated NRF2 skews fate trajectories of bone marrow cells from dendritic cells towards red pulp-like macrophages in hemolytic anemia.
    Cell Death Differ 29(8) 1450-1465(2022) 35031770