Strain Data Sheet

RBRC09601

Strain Information

Image
BRC No.RBRC09601
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Ccdc86<tm1Hfuj>
Former Common nameCyclon-KO(EN)
H-2 Haplotype
ES Cell lineiTL IC1 [C57BL/6NTac]
Background strain
Appearance
Strain developmentDeveloped by Hodaka Fujii, New York University in 2009. iTL IC1 ES cell line [C57BL/6NTac] was used. C57BL/6 background.
Strain descriptionCcdc86 (coiled-coil domain containing 86, Cyclon) knockout mice. EGFP and pgk-neo cassettes were placed just downstream of translation initiation codon in exon 1 of the gene. Heterozygous mice exhibited reduced Fas expression in activated T lyphocytes. Homozygous mutants are emobryonic lethal.
Colony maintenance
References
Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon.
Saint Fleur S, Hoshino A, Kondo K, Egawa T, Fujii H
Blood, 13;114(7), 1355-1365 (2009). 19528538

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ccdc86
MGI:1277220
coiled-coil domain containing 8619Ccdc86<tm1Hfuj>
MGI:4356276
targeted mutation 1, Hodaka Fujii
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)19EGFP
loxP phage P1 loxP19loxP
loxP phage P1 loxP19loxP
neo neomycin resistance gene (E. coli)19 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal T cell morphology (MP:0008037)

  • decreased T cell apoptosis (MP:0006414)

  • prenatal lethality, complete penetrance (MP:0011091)
  • Detailed phenotype data

    Ordering Information

    Donor DNAP1ファージ loxP, オワンクラゲ Green Fluorescent Protein (GFP)(緑色蛍光蛋白質), マウス Cyclon蛋白質遺伝子, マウス phosphoglycerate-kinase 1 (pgk) プロモーター, 大腸菌 ネオマイシン耐性遺伝子
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon. Saint Fleur S, Hoshino A, Kondo K, Egawa T, Fujii H. Blood (2009) 114 (7): 1355-65. The BIOLOGICAL RESOURCE shall be used only for academic research purposes by not-for-profit institutions. Use of the BIOLOGICAL RESOURCE by for-profit institutions or use for commercial research purposes shall require a commercial license from the Depositor. A recipient shall also acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of their use of the BIOLOGICAL RESOURCE.
    DepositorHodaka Fujii (New York University School of Medicine)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data