Strain Data Sheet

RBRC09609

Strain Information

Image
BRC No.RBRC09609
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Gfra1<tm2.1Jmi>
Former Common name
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype.
Uesaka T, Jain S, Yonemura S, Uchiyama Y, Milbrandt J, Enomoto H
Development, 134: 2171-2181(2007). 17507417

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site19Frt
GFP Green Fluorescent Protein (Aequorea victoria)19
Gfra1
MGI:1100842		glial cell line derived neurotrophic factor family receptor alpha 119Gfra1<tm2.1Jmi>
MGI:3715157		targeted mutation 2.1, Jeffrey Milbrandt
loxP phage P1 loxP19loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell death(MP:0000313)

  • abnormal cell morphology(MP:0000358)

  • abnormal enteric ganglia morphology(MP:0001045)

  • abnormal enteric nervous system morphology(MP:0001044)

  • abnormal nervous system development(MP:0003861)
    • more 5 phenotypes
  • abnormal neuronal precursor proliferation(MP:0004948)

  • absent enteric neurons(MP:0001048)

  • absent kidney(MP:0000520)

  • neonatal lethality, complete penetrance(MP:0011087)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, Jellyfish GFP cDNA, yeast FRT (flipase recombination target) site, mouse Gfra1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 134: 2171-2181(2007).
    DepositorHideki Enomoto (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data