Strain Data Sheet
RBRC09637
Strain Information | |
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| Image | |
| BRC No. | RBRC09637 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6-Gt(Rosa)26Sor<tm1.1(CAG-GCaMP6,-mCherry)Shi> |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J PLoS One, 7, e51286, (2012). 23240011 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| F2A | foot-and-mouth disease virus (FMD) 2A peptide | 6 | F2A | ||||
| GCaMP6 | RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | 6 | GCaMP6 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1.1(CAG-GCaMP6,-mCherry)Shi> | targeted mutation 1.1, RIKEN BRC | |||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 6 | WPRE | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | 6 | mCherry | ||||
| poly A | polyadenylic acid additional signal | 6 | poly A | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 LoxP site, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE), polyA, mouse Gt(Rosa)26Sor genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7(12) e51286 (2012). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR and the DEPOSITOR of mouse strain RBRC09450 using the Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR’s permission. |
| Depositor | RIKEN BRC (RIKEN BRC) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |