Strain Information | |
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Image | |
BRC No. | RBRC09657 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Clec4a4<tm1.1Ksat> |
Former Common name | Clec4a4-/- mice |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to C57BL/6J for 9 generations. |
Strain description | Clec4A4 (DCIR2) knockout mice. IRES-EGFP cassette was replaced with exon 1 and exon 2 of the Clec4A4 gene. No GFP expression was detected in the mice. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Nat. Commun., 7, 11273(2016). 27068492 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Clec4a4C-type lectin domain family 4, member a46Clec4a4<tm1.1Ksat>targeted mutation 1.1, Katsuaki Sato Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRES2internal ribosomal entry site (EMCV)6IRES2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), Aequorea Victoria EGFP cDNA, phage P1 loxP site, mouse Clec4a4 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 7, 11273 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Clec4a4-IRES2EGFP KI transgenic mice for Academic Research Purposes” specified by the DEPOSITOR and Inserm. |
Depositor | Katsuaki Sato (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |