Strain Data Sheet

RBRC09661

Strain Information

Image
BRC No.RBRC09661
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm12.1(Nphs1-EGFP)Maoh>
Former Common nameNephrin-EGFP mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Masato Ohtsuka and Shinji Hadano, Tokay University in 2012.
Strain descriptionKnock-in mice of a DNA construct, Nephrin-eGFP-poly A, at the Rosa26 locus. This strain was developed with the PITT method invented by depositors. The mice were originated from the E14 ES cell line (129P2) and then maintained in C57BL/6 background for more than 5 generations.
Colony maintenance
References
Establishment of Nephrin Reporter Mice and Use for Chemical Screening.
Tsuchida J, Matsusaka T, Ohtsuka M, Miura H, Okuno Y, Asanuma K, Nakagawa T, Yanagita M, Mori K
PLoS One, 11(6):e0157497 (2016). 27362433

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP mouse Nephrin promoter
Frt yeast FRT (flippase recombination target) site6Frt
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm12.1(Nphs1-EGFP)Maoh> targeted mutation 12.1, Masato Ohtsuka
SA Splice acceptor6SA
lox2272 phage P1 loxP variant6lox2272
SV40 polyA signal6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus Splicing Acceptor, yeast FRT site, Simian virus 40 (SV40) polyA, jellyfish eGFP cDNA, mouse Nephrin promoter, phage P1 loxP variant (lox2272), mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasato　Ohtsuka (Tokai University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data