Strain Data Sheet

RBRC09674

Strain Information

Image
BRC No.RBRC09674
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Atg13<em1Nmz>
Former Common nameAtg13 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. With the CRISPR/Cas9, a frameshift mutation was introduced into exon 5 of the Atg13 gene. C57BL/6 genetic background.
Strain descriptionAtg13 knockout mice. Homozygous mutants are lethal at embryonic day 17, showing myocardial growth defects.
Colony maintenance
ReferencesMol. Cell Biol., 36, 585-595 (2015). 26644405

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Atg13autophagy related 132Atg13<em1Nmz>endonuclease-mediated mutation 1, Noboru Mizushima

Ordering Information

Donor DNACRISPR法による遺伝子欠損のみ（外来遺伝子挿入無し）
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol. 36, 585-595 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Mizushima (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data