Strain Information | |
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Image | |
BRC No. | RBRC09688 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C.Cg-Siglech<tm1.1Ksat> |
Former Common name | Siglechdtr/dtr mice |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | BALB/cAJcl |
Appearance | |
Strain development | Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to BALB/c for 12 generations. |
Strain description | IRES-DTR-EGFP cassette were inserted into 3' untranslated region of Siglech, Siglech sialic acid binding Ig-like lectin H gene, highly expressed in plasmacytoid dendritic cells (pDCs). Homozygous mutant mice are defective in the transcriptional and cell surface expression of Siglech. Mice treated with diphtheria toxin (DT) cause specific ablation of pDCs. |
Colony maintenance | Homozygote x Homozygote [or Crossing to BALB/cAJcl] |
References | Immunity, 35, 958-971 (2011). 22177923 J. Allergy Clin. Immunol., (2018). 29477579 Sci. Rep., 6, 24477 (2016). 27075414 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)7EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBEGFheparin-binding EGF-like growth factor (human)7HBEGF Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRES2internal ribosomal entry site (EMCV)7IRES2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site2 (ires2), human HB-EGF cDNA, Aequorea Victoria EGFP cDNA, phage P1 loxP site, mouse Siglech genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 35, 958-971 (2011). Sci. Rep., 6, 24477 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes” specified by the DEPOSITOR and Inserm. |
Depositor | Katsuaki Sato (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:Depositor's present address; Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki. |
BRC mice in Publications |
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No Data |