Strain Information | |
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Image | |
BRC No. | RBRC09711 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6N-Tg(Arc-tTA/EGFP/Capzb<*>2)100Mkyph |
Former Common name | ACE-TG, AiCE-Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrlCrlj |
Appearance | |
Strain development | Developed by Maki Yamada, Tokushima Bunri University, Kagawa School of Pharmaceutical Sciences. A DNA construct was injected to fertilized eggs derived from C57BL/6N. Mice were maintained in C57BL/6 background. |
Strain description | Transgenic mice expressing GFP/Capzb2 fusion protein and tTA under the control of Arc gene promoter (7.0kb). This line has 100 copies of the TG construct. |
Colony maintenance | |
References | Sci. Rep., 10(1):15266 (2020). doi: 10.1038/s41598-020-72248-4. 32943708 Cerebral Cortex,19, 2572-2578 (2009). 19240139 Genes to Cells, 15, 737-747 (2010). 20545768 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Capzbcapping protein (actin filament) muscle Z-line, betaUNCapzb Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptideUNP2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAmouse Arc promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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Donor DNA | mouse Arc promoter, tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain; Clontech Tet-Off Advanced Inducible Gene Expression System), P2A (porcine teschovirus-1 2A peptide), jellyfish EGFP cDNA from Clontech EGFP-C1 vector, mouse CapZ cDNA, SV40 polyA signal from Clontech EGFP-C1 vector[update 07/14/2020] |
Research application | Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | 1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested, referring to the mice as "AiCE-Tg". Traceable stimulus-dependent rapid molecular changes in dendritic spines in the brain. Kuboyama K. et al. Scientific Reports 10 15266 (2020). DOI :10.1038/s41598-020-72248-4 https://www.nature.com/articles/s41598-020-72248-4 2, For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Maki Yamada (Tokushima Bunri University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Sequence of AiCE-Transgene |
BRC mice in Publications |
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No Data |