Strain Data Sheet

RBRC09735

Strain Information

Image
BRC No.RBRC09735
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)56Kenw
Former Common nameLGL-IL6 (Li56)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Ken Watanabe, National Center for Geriatrics and Gerontology in 2015. C57BL/6N genetic background.
Strain descriptionTransgenic mouse line to express Human IL-6 in a Cre-dependent manner. CAG promoter drives loxP-GFP-pA-loxP-IL-6-2A-mCherry-pA. Another transgenic subline of RBRC09735 is available as RBRC09734.
Colony maintenance
References
Generation of a transgenic mouse line for conditional expression of human IL-6.
Mori T, Murasawa Y, Ikai R, Hayakawa T, Nakamura H, Ogiso N, Niida S, Watanabe K
Exp. Anim., 65(4):455-463 (2016). 27349442

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
GH Growth hormone polyA (Bovine)UNGH
IL6 interleukin 6 (human)UNIL6
T2A Thosea asigna virus 2A peptide (insect)UNT2A
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
mCherry Red Fluorescent Protein (Discosoma sp.)UNmCherry
Mouse Proacrosin signal sequenceUN
Thy-1 GPI anchor signal sequenceUN
SV40 polyAUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage loxP sites, Aequorea victoria EGFP cDNA, SV40 PolyA signal, Human IL-6 cDNA, Mouse Proacrosin signal sequence; Thy-1 GPI anchor signal sequence, Thosea asigna virus 2A peptide (T2A), Discosoma sp. mCherry cDNA, Bovine GH polyA signal
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    covid19_onset
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mori et al. Exp. Anim. (2016)
    DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data