Strain Data Sheet

RBRC09746

Strain Information

Image
BRC No.RBRC09746
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Has1<tm1Nita>
Former Common nameHAS1 knockout mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Naoki Itano and Koji Kimata, Aichi Medical University in 2000. E14 ES cell was used. C57BL/6N congenic line.
Strain descriptionHas1 (hyaluronan synthase1) knockout mice.
Colony maintenance
References
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald J A, Kimata K, Taniguchi S, Itano N
Cancer Res., 70(18):7073-83 (2010). 20823158

Enhanced inflammation and accelerated wound closure following tetraphorbol ester application or full-thickness wounding in mice lacking hyaluronan synthases Has1 and Has3.
Mack J A, Feldman R J, Itano N, Kimata K, Lauer M, Hascall V C, Maytin E V
J. Invest. Dermatol., 132, 198-207 (2012). 21850020

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Has1
MGI:106590		hyaluronan synthase 117Has1<tm1Nita>
MGI:4835713		targeted mutation 1, Naoki Itano
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal17

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase (PGK) promoter, E coli Neomycin resistance gene, SV40 PolyA signal, Mouse Has1 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorKoji Kimata (Aichi Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data