Strain Data Sheet

RBRC09776

Strain Information

Image
BRC No.RBRC09776
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Efcab3<tm1a(KOMP)Osb>/40
Former Common nameEfcab3#40
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Miyata and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2012. C57BL/6N genetic background.
Strain descriptionFloxed Efcab3 line. A pair of loxP flanks Exon 6.
Colony maintenance
References
Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda J M, Fujihara Y, Yu Z, Archambeault D R, Isotani A, Kiyozumi D, Kriseman M L, Mashiko D, Matsumura T, Matzuk R M, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk M M
Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Efcab3
MGI:1918144		EF-hand calcium binding domain 311Efcab3<tm1a(KOMP)Osb>
MGI:4454078		targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)11En2
Frt yeast FRT (flippase recombination target) site11Frt
Frt yeast FRT (flippase recombination target) site11Frt
lacZ beta-galactosidase (E. coli)11
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
neo neomycin resistance gene (E. coli)11
poly A polyadenylic acid additional signal11poly A
poly A polyadenylic acid additional signal11poly A

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNA[chicken beta-actin promoter, rabbit beta-globin polyadenylation signal,CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter], Yeast FRT sites, mouse En2 splice acceptor (En2 intron2/exon 3 splice acceptor), EMCV Internal ribosomal entry site (IRES), E. coli. lacZ, SV40 polyadenylation site, Phage P1 loxP sites, human Beta-actin promoter, E. coli. Neomycin phosphotransferase gene (neoR) , mouse Efcab3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data