Strain Data Sheet

RBRC09788

Strain Information

Image
BRC No.RBRC09788
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Spink8<em1Osb> Spink8<em2Osb>
Former Common nameSpink8 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yuhkoh Satouh a and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. BDF1 background.
Strain descriptionSpink8 mutant mice generated by the CRISPR/Cas9 technique. Spink8<em1Osb> :[5 bp deletion in exon 1]tactgaagagaacagcatgtgactcgtgtctgttgcaggaacactgattctgcagatggttatcacagaaggaatttctcccccagagaacGGAGAGGGTAGGGGAGAACAGGAAAGACTACTTTTCTCTACACTGCGGGGGCCAGCAGAGATAGAGACGCTGCTGTCCTTCTGACGTCTTTAACACTGAGCTGAGGCTCCTTCAGTCAGGAGTGTCTTCCACAGGTGAAAGCAAGCAAAGCCAGAGGGCAAATAACTGCTTCCTTCCACAGGTCTGTCACCA[TGAAG]GTGATCTTCTCAGTTGCAGTCCTTGTTCTGGCCAGCTCAGTGTGGACTTCCCTTGCAGTTGgtgagttacaacttcttgctctatccacattttctgcaatatatctaatcgatggggatattagtggtataaatgccatgccattagattcattcgtttgttcactcattcatagtgctagagattgaataccttgtgcatattgcctaagcattgtaccatttagccatatccccagtcaccactttgatttatttgatccttaactcattttacaaatgaagcagaaatccagagaaagaaaagattcaSpink8<em2Osb>:(1 bp insertion at ATG codon of exon 1)tactgaagagaacagcatgtgactcgtgtctgttgcaggaacactgattctgcagatggttatcacagaaggaatttctcccccagagaacGGAGAGGGTAGGGGAGAACAGGAAAGACTACTTTTCTCTACACTGCGGGGGCCAGCAGAGATAGAGACGCTGCTGTCCTTCTGACGTCTTTAACACTGAGCTGAGGCTCCTTCAGTCAGGAGTGTCTTCCACAGGTGAAAGCAAGCAAAGCCAGAGGGCAAATAACTGCTTCCTTCCACAGGTCTGTCACCA(T)TGAAGGTGATCTTCTCAGTTGCAGTCCTTGTTCTGGCCAGCTCAGTGTGGACTTCCCTTGCAGTTGgtgagttacaacttcttgctctatccacattttctgcaatatatctaatcgatggggatattagtggtataaatgccatgccattagattcattcgtttgttcactcattcatagtgctagagattgaataccttgtgcatattgcctaagcattgtaccatttagccatatccccagtcaccactttgatttatttgatccttaactcattttacaaatgaagcagaaatccagagaaagaaaagattca
Colony maintenance
References
Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda J M, Fujihara Y, Yu Z, Archambeault D R, Isotani A, Kiyozumi D, Kriseman M L, Mashiko D, Matsumura T, Matzuk R M, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk M M
Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spink8
MGI:1925959		serine peptidase inhibitor, Kazal type 89Spink8<em2Osb> endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University
Spink8
MGI:1925959		serine peptidase inhibitor, Kazal type 89Spink8<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Spink8 gene
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data