Strain Information | |
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Image | |
BRC No. | RBRC09797 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-Ano6<tm1.2Naga> |
Former Common name | TMEM16F KO |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Shigekazu Ngata, Jun Suzuki and Toshihiro Fujii at Osaka University in 2013. Bruce 4 ES cell line was used. Exon 2 and Neomycin resistance gene were removed by crossing CAG-Cre mice. |
Strain description | Ano6(anoctamin 6, Tmem16f) knockout mice. |
Colony maintenance | |
References | Calcium-dependent phospholipid scrambling by TMEM16F. Suzuki J, Umeda M, Sims P J, Nagata S Nature, 468, 834-838 (2010). 21107324Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. Suzuki J, Fujii T, Imao T, Ishihara K, Kuba H, Nagata S J. Biol. Chem., 288, 13305-13316 (2013). 23532839 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Ano6 MGI:2145890 | anoctamin 6 | 15 | Ano6<tm1.2Naga> | targeted mutation 1.2, Shigekazu Nagata | |||
loxP | phage P1 loxP | 15 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, mouse Ano6 genome DNA |
Research application | Cre/loxP system Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 288, 13305-13316 (2013).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Osaka University) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |