Strain Data Sheet

RBRC09806

Strain Information

Image
BRC No.RBRC09806
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-cat,-EGFP)39Miya
Former Common nameCAG-CAT-EGFP mice, CAG-LSL-EGFP mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Jun-ich Miyazaki in 1999. Transgene was injected into BDF1, and then mice were backcrossed to C57BL/6 for more than 30 generations.
Strain descriptionTransgenic reporter mice for Cre recombinase. Floxed cat gene can be excised by Cre recombinase, resulting in EGFP expression.
Colony maintenance
References
A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination.
Kawamoto S, Niwa H, Tashiro F, Sano S, Kondoh G, Takeda J, Tabayashi K, Miyazaki J
FEBS Lett., 470, 263-268 (2000). 10745079

Health Report

Examination Date / Room / Rack2024/12/09Room:3-5Rack:DSentinel mouse program
2024/09/09Room:3-5Rack:DSentinel mouse program
2024/06/10Room:3-5Rack:DSentinel mouse program
2024/03/11Room:3-5Rack:DSentinel mouse program
2023/12/11Room:3-5Rack:DSentinel mouse program
2023/09/11Room:3-5Rack:DSentinel mouse program
2023/06/13Room:3-5Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
cat E. coli chloramphenicol acetyltransferase (CAT)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obese(MP:0001261)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli Chloramphenicol acetyltransferase gene, jellyfish Enhanced green fluorescent protein cDNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Letters, 470, 263-268 (2000). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for- profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJun-ichi Miyazaki (Osaka University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Baba Y, Onishi-Sakamoto S, Ide K, Nishifuji K.
    Nestin is a marker of unipotent embryonic and adult progenitors differentiating into an epithelial cell lineage of the hair follicles.
    Sci Rep 12(1) 17820(2022) 36280775