Strain Data Sheet

RBRC09815

Strain Information

Image
BRC No.RBRC09815
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-PcdhgC3<c.2400_2401del>PcdhgC4<c.2405_2418del, c.2422C>A>
Former Common namePcdhgC3 delta(-2)_gC4 delta(-14)c.2422C>A
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pcdhgc3
MGI:1935201		protocadherin gamma subfamily C, 318Pcdhgc3
Pcdhgc4
MGI:1935203		protocadherin gamma subfamily C, 418Pcdhgc4

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • neonatal lethality, complete penetrance(MP:0011087)

  • tremors(MP:0000745)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine betaGH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
    Research application
    Specific Term and ConditionsIf the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data