Strain Data Sheet

RBRC09821

Strain Information

Image
BRC No.RBRC09821
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Napepld<tm1.1Ueda>
Former Common nameNAPE-PLD floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dale G. Deutsch at Stony Brook University, Natsuo Ueda at Kagawa University. ES cells derived from 129 (iTL1) were used. After crossing with a Flp line, this strain was backcrossed to C57BL/6N for more than 12 generations. C57BL/6N congenic line.
Strain descriptionFloxed mice of Napepld(N-acyl phosphatidylethanolamine phospholipase D)gene. Exon 3 was flanked by loxP sites.
Colony maintenance
References
Enzymatic formation of N-acylethanolamines from N-acylethanolamine plasmalogen through N-acylphosphatidylethanolamine-hydrolyzing phospholipase D-dependent and -independent pathways.
Tsuboi K, Okamoto Y, Ikematsu N, Inoue M, Shimizu Y, Uyama T, Wang J, Deutsch D G, Burns M P, Ulloa N M, Tokumura A, Ueda N
Biochimica et Biophysica Acta, 1811, 565-577 (2011). 21801852

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site5Frt
Napepld
MGI:2140885		N-acyl phosphatidylethanolamine phospholipase D5Napepld<tm1.1Ueda> targeted mutation 1, Natsuo Ueda
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, S. cerevisiae FRT site, mouse Napepld genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochim Biophys Acta., 1811, 565-577 (2011).
    DepositorNatsuo Ueda (Kagawa University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data