Strain Data Sheet

RBRC09823

Strain Information

Image
BRC No.RBRC09823
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Tktl1<em1Osb>
Former Common nameTktl1<delta 11+4/Y>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ayako Isotani and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. BDF1 background.
Strain descriptionTktl1 mutant mice generated by the CRISPR/Cas9 technique. Tktl1<em1Osb> [Deleted sequence], (Inserted sequence): 11 bp deletion and 4 bp insertion at exon 1.GTTCTGCGGCACAACGTAGAGGAAGGATGTCGGAAGCTGAGGCAAGCA[GTGGGATGGCC](GCAT)CACAACGCTGGACCTGATGAGAAGACATTGCAGGTGTTGCGGGACATGGCCAACCGCCTGCGAATCCGTTCCATCAAGGCCACAAATTCCTCGACCACTAGgtaaaaggcctgggaaggccttaccatggggttctttggttgttctttctgaagctgtgaggtgaagggaacctgctgttatcctccattttctgccttctgggtgccacctactgcttctagatgcccatgagtttcattcctcttcagccctggtggcaggggtacacccctgactaattgtgaccaaagttctggaagcctataagacctccttagtaaggaaaaaatctagaacccagtcgggtgaatgagcagtgttccctggtatttatcaagctagctctagtttggcattttacctagaaatggtgttttgtgtataagcaattgcattggaattggggttggtcat
Colony maintenance
References
Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda J M, Fujihara Y, Yu Z, Archambeault D R, Isotani A, Kiyozumi D, Kriseman M L, Mashiko D, Matsumura T, Matzuk R M, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk M M
Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tktl1
MGI:1933244		transketolase-like 1XTktl1<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine betaGH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Tktl1 gene
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data