Strain Data Sheet

RBRC09859

Strain Information

Image
BRC No.RBRC09859
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CMV-cat,-SDHA<*>Y215F)1Hom
Former Common nameCATFL-SDHAY215F Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yoshimi Homma, Fukushima Medical University in 2012. C57BL/6 genetic background.
Strain descriptionTransgenic mice expressing mutated SDHA (Succinate dehydrogenase A, Y215F) in a Cre-dependent manner. Floxed cat (chloramphenicol acetyltransferase) gene and SDHA(Y215F) are driven by the CMV promoter.
Colony maintenance
References
Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components.
Ogura M, Yamaki J, Homma M K, Homma Y
Biochem. J., 447(2), 281-289 (2012). 22823520

Mitochondrial reactive oxygen species suppress humoral immune response through reduction of CD19 expression in B cells in mice.
Ogura M, Inoue T, Yamaki J, Homma M K, Kurosaki T, Homma Y
Eur. J. Immunol., 47, 406-418 (2017). 27883180

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)UNFLAG
SDHA succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (human)UNSDHA
cat E. coli chloramphenicol acetyltransferase (CAT)UN
loxP phage P1 loxPUNloxP CMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A
loxP phage P1 loxPUNloxP
poly A polyadenylic acid additional signalUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell activation(MP:0008217)

  • decreased B-2 B cell number(MP:0008207)

  • decreased IgM level(MP:0001806)

  • decreased immature B cell number(MP:0008215)

  • increased B-1a cell number(MP:0008167)
    • more 1 phenotypes
  • oxidative stress(MP:0003674)
    • Detailed phenotype data

    Ordering Information

    Donor DNACytomegalovirus CMV promoter, rabbit beta globin intron, Phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, rabbit beta globin polyA, Human SDHA gene cDNA, FLAG epitope tag(synthetic), pA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. immunol., 47, 406-418 (2017).
    DepositorYoshimi Honma (Fukushima Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data