Strain Information | |
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Image | |
BRC No. | RBRC09860 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.B6D2-Tg(Thy1-FUS<*>deltaNLS)1Dit |
Former Common name | deltaNLF-FUStg |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | A human FUS cDNA without a nuclear localization signal was inserted into the pTSC21k vector, which encodes the 8.1 kb murine Thy1.2 expression cassette. This construct was Not I digested to remove vector sequences, co-injected into the pronucleus of fertilized eggs from Slc:BDF1 strain mice. Founders were bred and backcrossed with C57Bl6/J mice to establish transgenic lines. |
Strain description | A transgenic (tg) mouse overexpressing exogenous FUS without a nuclear localization signal (ΔNLS-FUS) develop progressive spastic motor deficits and neuronal loss in the motor cortex. The ΔNLS-FUS protein was restricted to the cytoplasm and formed ubiquitin/p62-positive aggregates. Endogenous FUS expression, nuclear localization, and splicing activity were not altered, indicating that mislocated FUS is sufficient for proteinopathy. RNA-sequence analysis revealed specific transcriptome alterations, including genes regulating dynein-associated molecules and endoplasmic reticulum stress. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to C57BL/6JJcl] |
References | Brain. 2016 Sep;139(Pt 9):2380-94. doi: 10.1093/brain/aww161. Epub 2016 Jun 30. 27368346 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FUSfused in sarcomaUNFUS |
Ordering Information | |
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Donor DNA | マウス Thy-1 promoterとマウスThy-1のexon3,4, ヒト human FUS cDNA without a nuclear localization signalを挿入, マウス Thy-1exon3,4 |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Brain 2016 Sep;139(Pt 9):2380-94. doi: 10.1093/brain/aww161. Epub 2016 Jun 30. |
Depositor | Daisuke Ito (Keio University) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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