Strain Data Sheet

RBRC09861

Strain Information

Image
BRC No.RBRC09861
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Calcr<tm1c(KOMP)Sfuk>
Former Common nameCalcr-floxed mice
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by So-ichiro Fukada, Osaka University. EGR-G01(129S2×C57BL/6NCr)ES cells were used. Mice were backcrossed to C57BL/6 for more than 4 generations after crossing to a CAG-Flpe line.
Strain descriptionCalcr (calcitonin receptor) knockout mice.
Colony maintenance
References
Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche.
Yamaguchi M, Watanabe Y, Ohtani T, Uezumi A, Mikami N, Nakamura M, Sato T, Ikawa M, Hoshino M, Tsuchida K, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H, Fukada S
Cell Rep., 13, 302-314 (2015). 26440893

Cell Rep., 29(8):2154-2163 (2019). 31747590

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Calcr
MGI:101950		calcitonin receptor6Calcr<tm1c(KOMP)Sfuk> targeted mutation 1c
  • osteoporosis(MedGEN)
    • Frt yeast FRT (flippase recombination target) site6Frt
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flippase recombination target) site, phage P1 loxP sites, mouse Calcr genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Reports, 13, 302-314 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
    DepositorSoichirou Fukada (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data