Strain Data Sheet

RBRC09862

Strain Information

Image
BRC No.RBRC09862
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pgam5<tm1Hijo>
Former Common name
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. RENKA (C57BL/6) ES cells were used. C57BL/6 genetic background.
Strain descriptionPgam5(phosphoglycerate mutase family member 5) knockout mice.
Colony maintenance
References
Mitochondrial phosphoglycerate mutase 5 uses alternate catalytic activity as a protein serine/threonine phosphatase to activate ASK1.
Takeda K, Komuro Y, Hayakawa T, Oguchi H, Ishida Y, Murakami S, Noguchi T, Kinoshita H, Sekine Y, Iemura S, Natsume T, Ichijo H
Proc. Natl. Acad. Sci. USA, 106(30):12301-12305 (2009). 19590015

The Ablation of Mitochondrial Protein Phosphatase Pgam5 Confers Resistance Against Metabolic Stress.
Sekine S, Yao A, Hattori K, Sugawara S, Naguro I, Koike M, Uchiyama Y, Takeda K, Ichijo H
EBioMedicine, 5, 82-92 (2016). 27077115

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pgam5
MGI:1919792		phosphoglycerate mutase family member 55Pgam5<tm1Hijo> targeted mutation 1, Hidenori Ichijo
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circadian temperature homeostasis(MP:0011020)

  • abnormal glucose tolerance(MP:0005291)

  • decreased body fat mass(MP:0014143)

  • decreased body weight(MP:0001262)

  • decreased circulating triglyceride level(MP:0002644)
    • more 7 phenotypes
  • decreased food intake(MP:0011940)

  • enlarged liver(MP:0000599)

  • increased brown adipose tissue amount(MP:0000005)

  • increased insulin sensitivity(MP:0002891)

  • increased length of allograft survival(MP:0004751)

  • increased mitochondrial number(MP:0011628)

  • mortality/aging(MP:0010768)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse phosphoglycerate kinase 1 promoter, E.coli neomycin-resistance gene, mouse Pgam5 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHidenori Ichijo (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data