Strain Data Sheet

RBRC09863

Strain Information

Image
BRC No.RBRC09863
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Klhdc10<tm1Hijo>
Former Common nameKLHDC10 KO
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. RENKA (C57BL/6) ES cells were used. C57BL/6 genetic background.
Strain descriptionKlhdc10(kelch domain containing 10) knockout mice.
Colony maintenance
References
The Kelch repeat protein KLHDC10 regulates oxidative stress-induced ASK1 activation by suppressing PP5.
Sekine Y, Hatanaka R, Watanabe T, Sono N, Iemura S, Natsume T, Kuranaga E, Miura M, Takeda K, Ichijo H
Mol. Cell, 48(5):692-704 (2012). 23102700

KLHDC10 Deficiency Protects Mice against TNFalpha-Induced Systemic Inflammation.
Yamaguchi N, Sekine S, Naguro I, Sekine Y, Ichijo H
PLoS One, 11(9), e0163118 (2016). 27631783

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Klhdc10
MGI:1924038		kelch domain containing 106Klhdc10<tm1Hijo> targeted mutation 1, Hidenori Ichijo
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal body temperature homeostasis(MP:0001777)

  • abnormal survival(MP:0010769)

  • decreased circulating interleukin-6 level(MP:0008597)

  • decreased interleukin-6 secretion(MP:0008706)

  • increased cell death(MP:0012556)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse phosphoglycerate kinase 1 promoter, E.coli neomycin-resistance gene, mouse Klhdc10 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHidenori Ichijo (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data