Strain Data Sheet

RBRC09881

Strain Information

Image
BRC No.RBRC09881
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Tmem173<em1Tsse>
Former Common nameSTING KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Ken Takashima, Hiroyuki Oshiumi and Tsukasa Seya, Hokkaido University in 2015. C57BL/6 genetic background.
Strain descriptionTmem173 (transmembrane protein 173) mutants generated by the CRISPR/Cas9 technique. 5 bp deletion was introduced at exon 3.Tmem173<em1Tsse>: 5 bp deletion at exon 3.ATGGGACTAGCTGGGCTGGGAGACCCAAGgagatggatgactagtcaggaccttaggccctgaaggacaggagaaccccagaagcatagctgtggatttcttgatgtctacagatgccatactccaacctgcatccagccatcccacggcccagaggtcaccgctccaaatatgtagccctcatctttctggtggccagcctgatgatcctttgggtggcaaaggatccaccaaatcacactctgaagtacctagcacttcacctagcCTCGCacgaacttggactactgttgaaaaacctctgctgtctggctgaagagctgtgccatgtccagtccaggtaaccctctgtggtctccacgatgacttgatacccagcaagtatcacacccttgactcctaaccttggccttccctgaacatcatcagctgagttgggctgggatctgaggctaggctgtcccctgcaggtaccagggcagctactggaaggctgtgcgcgcctgcctgggatgccccatccactgtatggctatgattctactatcgtcttatttctatttcctccaaaacactgctgacatatacctcagttggatgtttGGCCTTCTGGTCCTCTATAAGTCCCTAAGC
Colony maintenance
References
STING in tumor and host cells cooperatively work for NK cell-mediated tumor growth retardation.
Takashima K, Takeda Y, Oshiumi H, Shime H, Okabe M, Ikawa M, Matsumoto M, Seya T
Biochem. Biophy. Res. Comm., 478, 1764-1771 (2016). 27608599

Health Report

Examination Date / Room / Rack2025/01/07Room:3-BRack:JSentinel mouse program
2024/10/07Room:3-BRack:JSentinel mouse program
2024/07/08Room:3-BRack:JSentinel mouse program
2024/04/08Room:3-BRack:JSentinel mouse program
2024/01/09Room:3-BRack:JSentinel mouse program
2023/10/10Room:3-BRack:JSentinel mouse program
2023/07/10Room:3-BRack:JSentinel mouse program
2023/04/10Room:3-BRack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tmem173
MGI:1919762		transmembrane protein 17318Tmem173<em1Tsse> endonuclease-mediated mutation 1, Tsukasa Seya
  • STING-associated vasculopathy with onset in infancy(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased NK T cell number(MP:0008040)

  • decreased double-positive T cell number(MP:0005092)

  • decreased interferon-alpha secretion(MP:0008563)

  • decreased interferon-beta secretion(MP:0008565)

  • decreased interferon-gamma secretion(MP:0008567)
    • more 1 phenotypes
  • increased tumor growth/size(MP:0003721)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9(Addgene plasmid #42230)[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR)**, E. coli pUC origin] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan).** E. coli Ampicillin resistance gene was not detected by PCR. Other introduced genes were not tested.
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorTsukasa Seya (Hokkaido University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Suzuki Y, Sato T, Sugimori M, Kanemaru Y, Onodera S, Tsuchiya H, Nakamori Y, Tsuyuki S, Ikeda A, Ikeda R, Goda Y, Kaneko H, Irie K, Sue S, Maeda S.
    Activation of STING in pancreatic cancer-associated fibroblasts exerts an antitumor effect by enhancing tumor immunity.
    Sci Rep 14(1) 17071(2024) 39048609
    Maekawa H, Inoue T, Ouchi H, Jao TM, Inoue R, Nishi H, Fujii R, Ishidate F, Tanaka T, Tanaka Y, Hirokawa N, Nangaku M, Inagi R.
    Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury.
    Cell Rep 29(5) 1261-1273.e6(2019) 31665638