Strain Data Sheet

RBRC09893

Strain Information

Image
BRC No.RBRC09893
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Crh<tm1.1(venus)Ksak>
Former Common namemCrh-Venus-deltaNeo
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kenji Sakimura at Niigata University, and Keiichi Itoi at Tohoku University. C57BL/6 background.
Strain descriptionVenus was knocked-in at exon 2 of Crh (Corticotropin releasing factor) gene. This strain was generated by crossing RBRC06519 with a Flpe line.
Colony maintenance
References
Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse.
Kono J, Konno K, Talukder A H, Fuse T, Abe M, Uchida K, Horio S, Sakimura K, Watanabe M, Itoi K
Brain Struct. Funct., 222, 1705-1732 (2017). 27638512

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:FSentinel mouse program
2024/10/21Room:3-CRack:FSentinel mouse program
2024/07/22Room:3-CRack:FSentinel mouse program
2024/04/22Room:3-CRack:FSentinel mouse program
2024/01/22Room:3-CRack:FSentinel mouse program
2023/10/23Room:3-CRack:FSentinel mouse program
2023/07/26Room:3-CRack:FAdditional tests
2023/07/24Room:3-CRack:FSentinel mouse program
2023/04/24Room:3-CRack:FSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Crh
MGI:88496		corticotropin releasing hormone3Crh<tm1.1(venus)Ksak> targeted mutation 1.1, Kenji Sakimura
Frt yeast FRT (flippase recombination target) site3Frt
Venus Yellow Fluorescent Protein (Aequorea victoria)3Venus

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAA. coerulescens Venus cDNA, yeast Frt (flippase recombination target) site, mouse Crh genomic DNA
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Brain Struct. Funct., 222, 1705-1732 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorKeiichi Itoi (Tohoku University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data