Strain Information | |
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Image | |
BRC No. | RBRC09893 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Crh<tm1.1(venus)Ksak> |
Former Common name | mCrh-Venus-deltaNeo |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Sakimura at Niigata University, and Keiichi Itoi at Tohoku University. C57BL/6 background. |
Strain description | Venus was knocked-in at exon 2 of Crh (Corticotropin releasing factor) gene. This strain was generated by crossing RBRC06519 with a Flpe line. |
Colony maintenance | |
References | Brain Struct. Funct., 222, 1705-1732 (2017). 27638512 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/22Room:3-CRack:F 2023/10/23Room:3-CRack:F 2023/07/26Room:3-CRack:F 2023/07/24Room:3-CRack:F 2023/04/24Room:3-CRack:F 2023/01/23Room:3-CRack:F 2022/10/25Room:3-CRack:F 2022/07/25Room:3-CRack:F 2022/04/25Room:3-CRack:F |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)3Venus |
Ordering Information | |
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Donor DNA | A. coerulescens Venus cDNA, yeast Frt (flippase recombination target) site, mouse Crh genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Brain Struct. Funct., 222, 1705-1732 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Keiichi Itoi (Tohoku University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |