Strain Data Sheet

RBRC09894

Strain Information

Image
BRC No.RBRC09894
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Has2<tm1.1Nita>
Former Common nameHas2 conditional knockout
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naoki Itano, Kyoto Sangyo University in 2006. An ES cell line derived from 129/SvEvTac was used. This line was crossed with a CAG-FLPe line to remove FRT-flanked neo cassette, and then backcrossed to C57BL/6 for 7 generations.
Strain descriptionFloxed Has2 mice. Homozygous null mutants are embyonically lethal.
Colony maintenance
References
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald J A, Kimata K, Taniguchi S, Itano N
Cancer Res., 70(18), 7073-7083（2010）. 20823158

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site15Frt
Has2
MGI:107821		hyaluronan synthase 215Has2<tm1.1Nita>
MGI:4835686		targeted mutation 1.1, Naoki Itano
loxP phage P1 loxP15loxP
loxP phage P1 loxP15loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT recombinase target site, mouse Has2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorNaoki Itano (Kyoto Sangyo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data