Strain Data Sheet

RBRC09904

Strain Information

Image
BRC No.RBRC09904
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Has1<tm1Nita>Has2<tm1.1Nita>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naoki Itano, Kyoto Sangyo Universit. RBRC09746 and RBRC09894 are crossed.
Strain descriptionDouble mutant of Has1 KO and floxed Has2. Single mutant of Has1 KO or floxed Has2 is available as RBRC09746 or RBRC09894, respectively.
Colony maintenance
References
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald J A, Kimata K, Taniguchi S, Itano N
Cancer Res., 70(18), 7073-7083（2010）. 20823158

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site15Frt
Has2
MGI:107821		hyaluronan synthase 215Has2<tm1.1Nita>
MGI:4835686		targeted mutation 1.1, Naoki Itano
loxP phage P1 loxP15loxP
loxP phage P1 loxP15loxP
Has1
MGI:106590		hyaluronan synthase 117Has1<tm1Nita>
MGI:4835713		targeted mutation 1, Naoki Itano
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal17

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase (PGK) promoter, E col. Neomycin resistance gene, SV40 PolyA signal, mouse Has1 genomic DNA, Phage P1 loxP sites, yeast FRT recombinase target site, mouse Has2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorNaoki Itano (Kyoto Sangyo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data