Strain Data Sheet

RBRC09905

Strain Information

Image
BRC No.RBRC09905
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Anxa10<em1Siag>
Former Common nameAnxa10 -/-
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Shinji Sasaki, Shirakawa Institute of Animal Genetics, Japan Livestock Technology Association in 2016. This strain was generated by the CRISPR/CAS9 technique. B6D2 background.
Strain descriptionAnxa10 (annexin A10) mutant mice. 8 bp deletion at exon 2. Anxa10<em1Siag>: 8 bp deletion at exon 2.tttcatggaaattcatacaatctcttaagtgttgatttttttttcccgacagGTCCAAGGAACCATCTTCCCAGCTCCAAATTTCAATCCCATGATGGATGCCCAAATGCTAGGCGGAGCACTCCAAGGATTTGgtaagccttggaaatacattcgttgattatgaaaaaatataacgtaaaagtaaatttctaggcctttaggcccaggcttgagaatgtgacctctatgactcaatgctccaaggt
Colony maintenance
References
Loss of maternal ANNEXIN A10 via a 34-kb deleted-type copy number variation is associated with embryonic mortality in Japanese Black cattle.
Sasaki S, Ibi T, Akiyama T, Fukushima M, Sugimoto Y
BMC Genomics, 17, 968- (2016). 27881083

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Anxa10
MGI:1347090		annexin A108Anxa10<em1Siag> endonuclease-mediated mutation 1, Shinji Sasaki

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryo phenotype(MP:0005380)

  • maternal effect(MP:0003718)

  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine betaGH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. BMC Genomics, 17, 968- (2016).
    DepositorYoshikazu Sugimoto (Japan Livestock Technology Association)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data