Strain Data Sheet

RBRC09908

update : 2025-02-20

Strain Information
Image
BRC No.	RBRC09908
Type	Targeted Mutation
Species	Mus musculus
Strain name	C.Cg-Braf<tm1Tumg>
Former Common name	Braf Q241R Neo/+ (BALB/c background)
H-2 Haplotype
ES Cell line	Bruce 4 [B6.Cg-Thy1<a>]
Background strain	BALB/cAnNCrlCrlj
Appearance
Strain development	eveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. B6.Cg-Braf<tm1Tumg>(RBRC09730) was backcrossed to BALB/c for 10 generations.
Strain description	Knock-in mutants in which Braf-mutated protein (BrafQ241R) is expressed in a Cre-dependent manner. BALB/c genetic background. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. Strains maintained in B6 and 129 genetic background are available as RBRC09730 and RBRC09907, respectively. Mice constitutively expressing BrafQ241R in the ICR genetic background are available as RBRC09731.
Colony maintenance
References	New BRAF knockin mice provide a pathogenetic mechanism of developmental defects and a therapeutic approach in cardio-facio-cutaneous syndrome. Inoue S, Moriya M, Watanabe Y, Miyagawa-Tomita S, Niihori T, Oba D, Ono M, Kure S, Ogura T, Matsubara Y, Aoki Y Hum. Mol. Genet., 23, 6553-6566 (2014). 25035421

Health Report
Examination Date / Room / Rack	2024/11/25Room:4-BRack:HSentinel mouse program 2024/08/26Room:4-BRack:HSentinel mouse program 2024/05/27Room:4-BRack:HSentinel mouse program

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Braf MGI:88190	Braf transforming gene	6	Braf<tm1(Q241R)Tumg>	targeted mutation 1, Yoko Aoki			cardiofaciocutaneous syndrome 1(MedGEN) cardiofaciocutaneous syndrome(DisGeNET) colorectal cancer(MedGEN) more 8 Diseases Langerhans cell histiocytosis(DisGeNET) LEOPARD syndrome 3(MedGEN) lung cancer(MedGEN) melanoma(DisGeNET) melanoma, cutaneous malignant, susceptibility to, 1(MedGEN) Noonan syndrome 1(MedGEN) Noonan syndrome 7(MedGEN) thyroid gland papillary carcinoma(DisGeNET)
Frt	yeast FRT (flippase recombination target) site	6	Frt
Frt	yeast FRT (flippase recombination target) site	6	Frt
loxP	phage P1 loxP	6	loxP
loxP	phage P1 loxP	6	loxP
neo	neomycin resistance gene (E. coli)	6				mouse phosphoglycerate kinase promoter (PGK promoter)
	SV40 polyA signal	6

Phenotype
Annotation by Mammalian phenotyhpe ontology	no abnormal phenotype detected(MP:0002169)
Detailed phenotype data

Ordering Information
Donor DNA	Bacteriophage P1 loxP sites, STOP transcriptional sequence (Adenovirus splice acceptor, SV40 polyA), Saccharomyces cerevisiae FRT sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli　Neomycin resistant gene, Polyomavirus SV40 polyA signal, mouse Braf genomic DNA
Research application	Cre/loxP system FLP/frt system
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 23, 6553-6566 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Yoko Aoki (Tohoku University)
Strain Status	Live mice Frozen embryos Frozen sperm
Strain Availability	Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months)
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR- Mouse of the Month Nov 2022

BRC mice in Publications

No Data