Strain Data Sheet

RBRC09910

Strain Information

Image
BRC No.RBRC09910
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sik3<tm1.1Iiis>
Former Common nameSik3<Slp>
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NJcl
Appearance
Strain developmentWe induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities through EEG/EMG-based sleep staging. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing of Sleepy mutants identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. To confirm genetically that the Sik3 splice mutation is the sole cause of the long-sleep phenotype, we introduced the Sik3 exon 13-skipping allele into wild-type mice by using conventional knock-in in embryonic stem (ES) cells. As expected, these mouse lines exhibited markedly reduced wake time, similar to the original Sleepy pedigree.
Strain descriptionThis mouse line exhibited markedly reduced wake time.
Colony maintenance
References
Forward-genetics analysis of sleep in randomly mutagenized mice.
Funato H, Miyoshi C, Fujiyama T, Kanda T, Sato M, Wang Z, Ma J, Nakane S, Tomita J, Ikkyu A, Kakizaki M, Hotta-Hirashima N, Kanno S, Komiya H, Asano F, Honda T, Kim S J, Harano K, Muramoto H, Yonezawa T, Mizuno S, Miyazaki S, Connor L, Kumar V, Miura I, Suzuki T, Watanabe A, Abe M, Sugiyama F, Takahashi S, Sakimura K, Hayashi Y, Liu Q, Kume K, Wakana S, Takahashi J S, Yanagisawa M
Nature, 539, 378-383 (2016). 27806374

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site9Frt
Sik3
MGI:2446296		SIK family kinase 39Sik3<tm1.1Iiis>
MGI:5806542		targeted mutation 1.1, Masashi Yanagisawa and Hiromasa Funato
  • spondyloepimetaphyseal dysplasia, Krakow type(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal sleep pattern(MP:0001501)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site, mouse Sik3 genomic DNA
    Research applicationFLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from　Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorDrs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Mar 2019

    BRC mice in Publications

    No Data