Strain Data Sheet

RBRC09912

Strain Information

Image
BRC No.RBRC09912
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sik3<em3Iiis>
Former Common nameSik3<Flag,Slp>
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Hiromasa Funato and Masashi Yanagisawa, University of Tsukuba. C57BL/6N background. Flag-tag was inserted into RBRC09910 by the CRISPR/Cas9 technology.
Strain descriptionMouse line harboring a Flag-tag inserted into the N terminus of mutated SIK3 found in the Sleepy (Slp) mutant. Heterozygous males and females are prone to be poor breeders.
Colony maintenance
References
Forward-genetics analysis of sleep in randomly mutagenized mice.
Funato H, Miyoshi C, Fujiyama T, Kanda T, Sato M, Wang Z, Ma J, Nakane S, Tomita J, Ikkyu A, Kakizaki M, Hotta-Hirashima N, Kanno S, Komiya H, Asano F, Honda T, Kim S J, Harano K, Muramoto H, Yonezawa T, Mizuno S, Miyazaki S, Connor L, Kumar V, Miura I, Suzuki T, Watanabe A, Abe M, Sugiyama F, Takahashi S, Sakimura K, Hayashi Y, Liu Q, Kume K, Wakana S, Takahashi J S, Yanagisawa M
Nature, 539, 378-383 (2016). 27806374

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)9FLAG
Frt yeast FRT (flippase recombination target) site9Frt
HA human influenza virus HA (hemagglutinin) tag sequence9HA
Sik3
MGI:2446296		SIK family kinase 39Sik3<em3Iiis>
MGI:5806545		endonuclease-mediated mutation 3, Masashi Yanagisawa and Hiromasa Funato
  • spondyloepimetaphyseal dysplasia, Krakow type(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal circadian sleep/wake cycle(MP:0020478)

  • abnormal non-rapid eye movement sleep pattern(MP:0012289)

  • increased susceptibility to age related obesity(MP:0003212)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], mouse Sik3 genomic DNA, Synthetic FLAG tag, Human Influenza HA tag (Hemagglutinin gene), yeast FRT (flipase recombination target) site
    Research applicationFLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from　Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorDrs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Mouse of the Month Mar 2019

    BRC mice in Publications

    No Data