Strain Data Sheet

RBRC09913

Strain Information

Image
BRC No.RBRC09913
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Drd1<tm1.1Mmsh>
Former Common nameDrd1a floxed, B6-Drd1a floxed, fDrd1a
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Noboru Mizushima, The University of Tokyo. RENKA ES cells derived from C57BL/6N was used and mice were maintained by brother-sister mating. A neo cassette was removed by crossing with B6-Tg(CAG-FLPe)36.
Strain descriptionFloxed Drd1a mice. Exon 2 of Drd1 is flanked by loxP sites. Conditional Drd1 deficient mice can be generated by crossing with cell type-specific Cre driver mice.
Colony maintenance
References
Striatal dopamine D1 receptor is essential for contextual fear conditioning.
Ikegami M, Uemura T, Kishioka A, Sakimura K, Mishina M
Sci. Rep., 4:3976 (2014). 24496082

Health Report

Examination Date / Room / Rack2024/06/10Room:3-5Rack:DSentinel mouse program
2024/03/11Room:3-5Rack:DSentinel mouse program
2023/12/11Room:3-5Rack:DSentinel mouse program
2023/09/11Room:3-5Rack:DSentinel mouse program
2023/06/13Room:3-5Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Drd1
MGI:99578		dopamine receptor D113Drd1<tm1.1Mmsh>
MGI:5617500		targeted mutation 1.1, Masayoshi Mishina
Frt yeast FRT (flippase recombination target) site13Frt
loxP phage P1 loxP13loxP
loxP phage P1 loxP13loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • behavior/neurological phenotype(MP:0005386)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Drd1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR during first TWO years after deposition by the DEPOSITOR to the RIKEN BRC. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasayoshi Mishina (Ritsumeikan University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data