Strain Data Sheet

RBRC09920

Strain Information

Image
BRC No.RBRC09920
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pdx1<em1(cre/ERT2)Rbrc>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentRIKEN BRC developed this strain by the CRISPR/Cas9 technique using fertilized eggs derived from C57BL/6NJcl. This was funded through the FY2016 AMED-NBRP fundamental technology upgrading program.
Strain descriptionCre/ERT2 knockin mice at the Pdx1 gene locus. A 2A-Cre/ERT2 DNA fragment was placed in frame at the C-terminus of the Pdx1 protein coding sequence.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)5ERT2
HBB2 rabbit beta-globin polyA5HBB2
P2A porcine teschovirus-1 2A peptide5P2A
Pdx1
MGI:102851		pancreatic and duodenal homeobox 15Pdx1<em1(cre/ERT2)Rbrc> endonuclease mediated mutation 1, RIKEN BRC
  • maturity-onset diabetes of the young type 4(MedGEN)

  • maturity-onset diabetes of the young(DisGeNET)

  • pancreatic agenesis 1(MedGEN)
    • more 1 Diseases
  • type 2 diabetes mellitus(MedGEN)
    • cre Phage P1 Cre recombinase5cre

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), Bacteriophage P1 Cre, Human ERT2 cDNA, Rabbit Beta globin polyA, mouse Pdx1 genomic DNA
    Research applicationCre/loxP system
    Cre driver
    Specific Term and Conditions1, In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to RIKEN BRC is requested.
    2, For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    3, RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data