Strain Data Sheet

RBRC09921

Strain Information

Image
BRC No.RBRC09921
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Trp53<em1Rbrc>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentRIKEN BRC developed this strain by the CRISPR/Cas9 technique using fertilized eggs derived from C57BL/6NJcl. This was funded through the FY2016 AMED-NBRP fundamental technology upgrading program.
Strain descriptionTrp53 flox mice in the C57BL/6N genetic background. A pair of loxP sites flanks a genomic region from Exon 2 to Exon 9 of the Trp53 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/12/09Room:3-3Rack:BSentinel mouse program
2024/09/09Room:3-3Rack:BSentinel mouse program
2024/06/10Room:3-3Rack:BSentinel mouse program
2024/03/11Room:3-3Rack:BSentinel mouse program
2023/12/11Room:3-3Rack:BSentinel mouse program
2023/09/11Room:3-3Rack:BSentinel mouse program
2023/06/13Room:3-3Rack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53
MGI:98834		transformation related protein 5311Trp53<em1Rbrc> endonuclease mediated mutation 1, RIKEN BRC
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
    • more 14 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)
    • loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse Trp53 genomic DNA
    Research applicationCre/loxP system
    Specific Term and Conditions1, In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to RIKEN BRC is requested.
    2, For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    3, RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Enomoto Y, Katsura H, Fujimura T, Ogata A, Baba S, Yamaoka A, Kihara M, Abe T, Nishimura O, Kadota M, Hazama D, Tanaka Y, Maniwa Y, Nagano T, Morimoto M.
    Autocrine TGF-β-positive feedback in profibrotic AT2-lineage cells plays a crucial role in non-inflammatory lung fibrogenesis.
    Nat Commun 14(1) 4956(2023) 37653024