Strain Data Sheet

RBRC09932

Strain Information

Image
BRC No.RBRC09932
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Prm2<em2(S56D)Kyot>
Former Common namePrm2 S56D
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Itoh, Graduate School of Medicine and Faculty of Medicine, Kyoto University in 2015. C57BL/6 genetic background.
Strain descriptionA point mutation S56D was intrduced into the Prm2 (protamine 2) gene by the CRISPR/Cas9 technique. Homozygous mice are fertile.
Colony maintenance
References
Dephosphorylation of protamine 2 at serine 56 is crucial for murine sperm maturation in vivo.
Itoh K, Kondoh G, Miyachi H, Sugai M, Kaneko Y, Kitano S, Watanabe H, Maeda R, Imura A, Liu Y, Ito C, Itohara S, Toshimori K, Fujita J
Sci. Signal., 12(574) (2019). 30914484

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prm2
MGI:97766		protamine 216Prm2<em1(S56A)Kyot> endonuclease-mediated mutation 1, Katuhiki Itoh

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
    Research application
    Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. 　The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Itoh (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data