Strain Data Sheet
RBRC09994
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC09994 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(Eno2-GFP/Atg5)1Nmz |
| Former Common name | Nse-Atg5 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background. |
| Strain description | Transgenic mice expressing GFP-Atg5 fusion protein under the control of the rat Eno2/Nse(neuron specific enolase)promoter. |
| Colony maintenance | |
| References | Dev. Cell, 39, 116-130 (2016). 27693508 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPrat Eno2 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)UNSV40 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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| Donor DNA | Rat Neuron specific enolase promoter, jellyfish Green fluorescent protein (GFP) cDNA, mouse Atg5 cDNA, SV40 polyA additional signal, SV40 early promoter, E. coli neomycin resistance gene |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell 39: 116-130 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 1. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. 2. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 3. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Noboru Mizushima (The University of Tokyo) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |