Strain Data Sheet

RBRC10003

Strain Information

Image
BRC No.RBRC10003
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;Cg-Hsf1<tm1Anak>
Former Common nameHSF1KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Sachiye Inoue and Akira Nakai, Yamaguchi University in 2003. TT2 ES cells(C57BL/6 x CBA F1 origin)were used. Mice were backcrossed to ICR.
Strain descriptionHsf1 (heat shock transcription factor 1) knockout mice. Homozygous females are infertile.
Colony maintenance
References
Activation of heat shock genes is not necessary for protection by heat shock transcription factor 1 against cell death due to a single exposure to high temperatures.
Inouye S, Katsuki K, Izu H, Fujimoto M, Sugahara K, Yamada S, Shinkai Y, Oka Y, Katoh Y, Nakai A
Mol. Cell Biol., 23, 5882-5895 (2003). 12897157

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hsf1
MGI:96238		heat shock factor 115Hsf1<tm1Anak>
MGI:3044161		targeted mutation 1, Akira Nakai
neo neomycin resistance gene (E. coli)15 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal spermatogenesis(MP:0001156)

  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase I (PGK) promoter, E. coli neomycin resistance gene, mouse phosphoglycerate kinase I (PGK) polyA signal, mouse Hsf1 genomic DNA
    Research applicationCancer Research
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 23, 5882-5895 (2003). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAkira Nakai (Yamaguchi University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jul 2019

    BRC mice in Publications

    No Data