Strain Data Sheet

RBRC10016

Strain Information

Image
BRC No.RBRC10016
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Rhoj<tm1Maim>/76Maim
Former Common name
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by a group at Nagoya City University and Osaka University at 2006. D3 ES cell line was used. Mice were backcrossed to C57BL/6J for 18 generations. C57BL/6 congenic strain.
Strain descriptionRhoj(ras homolog family member J, TCL/TC10bL)knockout mice.
Colony maintenance
References
Crucial role of TCL/TC10beta L, a subfamily of Rho GTPase, in adipocyte differentiation.
Nishizuka M, Arimoto E, Tsuchiya T, Nishihara T, Imagawa M
J. Biol. Chem., 278, 15279-15284 (2003). 12578843

TC10-like/TC10betaLong regulates adipogenesis by controlling mitotic clonal expansion.
Kawaji A, Nishizuka M, Osada S, Imagawa M
Biol. Pharm. Bull., 33, 404-409 (2010). 20190400

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rhoj
MGI:1931551		ras homolog family member J12Rhoj<tm1 Maim> targeted mutation 1, Masayoshi Imagawa
neo neomycin resistance gene (E. coli)12 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomysin resistant gene, mouse PGK polyA, mouse Rhoj genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorMakoto Nishizuka (Nagoya City University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data