Strain Data Sheet

RBRC10017

Strain Information

Image
BRC No.RBRC10017
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Lcmt1<tm1d(EUCOMM)Hmgu>/C09
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NJcl
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site7Frt
Frt yeast FRT (flippase recombination target) site7Frt
Lcmt1
MGI:1353593		leucine carboxyl methyltransferase 17Lcmt1<tm1e(EUCOMM)Hmgu>
MGI:4455788		targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH
lacZ beta-galactosidase (E. coli)7
loxP phage P1 loxP7loxP
loxP phage P1 loxP7loxP
neo neomycin resistance gene (E. coli)7

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Lcmt1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data