Strain Data Sheet

RBRC10052

Strain Information

Image
BRC No.RBRC10052
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-S1pr2<tm1.1Toka>
Former Common nameS1pr2-venus
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takaharu Okada, RIKEN Research Center for Allergy and Immunology around 2009-2011. B6N/B6J F1-hybrid ES cells were used and mice were backcrossed to C57BL/6J for 4 generations. C57BL/6 genetic background.
Strain descriptionThe first 498 bp of the S1pr2 protein coding region was replaced with the Venus gene. A loxP-flanked PGK-Neo cassette was inserted before exon 2, and was deleted later. S1pr2 gene expression can be analyzed by flow cytometry. Fluorescence of Venus in some cells such as germinal center B cells is detectable by microscopy but is not very bright unless cells/tissues are fixed and stained with anti-GFP and a fluorescent dye-labeled secondary antibody. Homozygous mice are S1pr2-deficient. About 40 % of homozygous mutants die around 3-5 weeks old.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl]
References
Sphingosine-1-phosphate receptor 2 is critical for follicular helper T cell retention in germinal centers.
Moriyama S, Takahashi N, Green J A, Hori S, Kubo M, Cyster J G, Okada T
J. Exp. Med., 211(7), 1297-1305 (2014). 24913235

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
S1pr2
MGI:99569		sphingosine-1-phosphate receptor 29S1pr2<tm1.1Toka>
MGI:5605543		targeted mutation 1.1, Takaharu Okada
  • autosomal recessive nonsyndromic hearing loss 68(DisGeNET, MedGEN)
    • Venus Yellow Fluorescent Protein (Aequorea victoria)9Venus
    loxP phage P1 loxP9loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • impaired hearing(MP:0006325)

  • premature death(MP:0002083)

  • torticollis(MP:0030126)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEnterobacteria phage P1 LoxP site, Aequorea victoria GFP variant (Venus) cDNA, mouse S1pr2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 211(7), 1297-1305 (2014). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTakaharu Okada (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data