Strain Data Sheet

RBRC10054

Strain Information

Image
BRC No.RBRC10054
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Aqp4<tm1Tsna>
Former Common nameAQP4 Neo, Aqp4 flox
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Mika Tsujita, Brain Research Institute, Niigata University. RENKA ES cells derived from C57BL/6 were used. C57BL/6 genetic background.
Strain descriptionAqp4 mutant mice in which loxP-FRT-pgk-neo-FRT DNA fragment was inserted between exon 1 and exon 2, and another loxP was inserted between exon 3 and exon 4.
Colony maintenance
References
Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice.
Kitaura H, Tsujita M, Huber V J, Kakita A, Shibuki K, Sakimura K, Kwee I L, Nakada T
Neurosci. Res., 64, 208-212 (2009). 19428702

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aqp4
MGI:107387		aquaporin 418Aqp4<tm1Tsna>
MGI:3848918		targeted mutation 1, Tsutomu Nakada
Frt yeast FRT (flippase recombination target) site18Frt
Frt yeast FRT (flippase recombination target) site18Frt
loxP phage P1 loxP18loxP
loxP phage P1 loxP18loxP
neo neomycin resistance gene (E. coli)18 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal18

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, Saccharomyces cerevisiae FRT sites, mouse Pgk1 promoter, E. coli aminoglycoside 3'-phosphotransferase, Simian virus 40 SV40 pA, mouse Aqp4 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 64, 208-212 (2009).
    DepositorMika Terumitsu-Tsujita (Niigata University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2017

    BRC mice in Publications

    No Data