Strain Data Sheet

RBRC10056

Strain Information

Image
BRC No.RBRC10056
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Aqp1<tm1Tsna>
Former Common nameAQP1 Neo
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Mika Tsujita, Brain Research Institute, Niigata University. RENKA ES cells derived from C57BL/6 were used. C57BL/6 genetic background.
Strain descriptionAqp1 mutant mice in which FRT-loxP-pgk-neo-FRT DNA fragment was inserted between exon 3 and exon 4, and another loxP was inserted between exon 1 and exon 2.
Colony maintenance
References
Virchow-Robin space and aquaporin-4: new insights on an old friend.
Nakada T
Croat. Med. J., 55(4), 328-336 (2014). 25165047

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aqp1
MGI:103201		aquaporin 16Aqp1<tm1Tsna> targeted mutation 1, Tsutomu Nakada
Frt yeast FRT (flippase recombination target) site6Frt
Frt yeast FRT (flippase recombination target) site6Frt
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) sites, mouse Pgk1 promoter, E. coli Aminoglycoside 3’ -phosphotransferase, SV40 polyA signal, mouse Aqp1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Croat. Med. J., 55(4), 328-336 (2014).
    DepositorMika Terumitsu-Tsujita (Niigata University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data